Answer:

Enzyme activity can be increased by a variety of methods. According to the search results provided on 2023-05-28, these include optimal levels of acidity, temperature, roughness, and composition. However, there is also another factor which affects the activity of an enzyme which is not listed above, and that factor is the presence of inhibitors.

Temperature

Temperature is one of the most important factors that affects the activity of an enzyme. According to PMC, the effect of temperature on enzyme activity can result thermal denaturation which is time dependent. As such, enzymes often have an optimal temperature at which they function best.

Acidity

The level of acidity can also affect the activity of an enzyme. According to Varsity Tutors, enzymes have optimal levels of acidity at which they function best.

Roughness and Composition

The roughness and composition of the environment in which the enzyme is present can also affect its activity. According to ScienceDirect, fold-increases noted in enzyme activity could result from changes in these factors.

Inhibitors

The presence of inhibitors is another factor which affects the activity of an enzyme. Inhibitors are molecules which bind to enzymes and decrease the rate of reaction. As such, inhibitors can also decrease the activity of an enzyme, making them a factor not included in the list above.

In conclusion, temperature, acidity, roughness, and composition can all increase the activity of an enzyme. However, inhibitors are another factor which affects the activity of an enzyme, and is not included in the list above.